Super-Resolution Fluorescence Microscopy The advent of super-resolution techniques is partly due to the wave nature of light. The resolution of light microscopy is limited because light is a wave and is subject to diffraction. This diffraction limit of light microscopy, about 200–300 nm in the lateral direction and 500-700 nm in the axial direction, is larger than many subcellular structures, leaving them too small to be observed in detail. Super-resolution techniques aim at overcoming this diffraction barrier, and include techniques that employ nonlinear effects to sharpen the point-spread function (PSF) of the microscope.
Super-Resolution Fluorescence Microscopy Lasers
635 nm Collimated Diode Laser System 5 - 500 mW Output Power