Application > Microscopy - Total Internal Reflection Fluorescence (TIRF)

Total Internal Reflection Fluorescence (TIRF)


Total Internal Reflection Fluorescence (TIRF) It is difficult to overestimate the impact fluorescence imaging has had on life sciences. Fluorescence has advanced the field dramatically, and has become the basis for numerous bio-imaging methods and applications. In cellular and molecular biology a large number of molecular-level events surrounding cellular surfaces, such as adhesion, binding of cells by way of hormones, secretion of neurotransmitters, and membrane dynamics have been studied with conventional fluorescence microscopes. Fluorophores that are bound to a specimen's surface provide us a great deal of information about it. The resulting fluorescence from these fluorophores is very low, compared to that of non-bound particles. Total Internal Reflection Fluorescence Microscopy (TIRFM) allows for selective excitation of the surface-bound fluorophores, while non-bound molecules are not excited and do not fluoresce. Due to the sub-micron surface selectivity of surface-bound fluorophores TIRFM has become the method of choice for in vitro single molecule detection and imaging.

Total Internal Reflection Fluorescence (TIRF) Lasers


Product WavelengthSpec Sheet Description Starting At In Stock
405 nm

Best Seller!

405 nm Collimated Diode Laser System
5 - 500 mW Output Power
$982.00
532 nm

Best Seller!

532 nm DPSS Laser System
5 - 300 mW Output Power
$1,004.00
635 nm

Best Seller!

635 nm Collimated Diode Laser System
5 - 500 mW Output Power
$1,037.00
635 nm

Best Seller!

635 nm Collimated Diode Laser System with Near-TEM00 Beam
5 - 200 mW Output Power
$1,187.00
1064 nm

Best Seller!

1064 nm Low-Noise DPSS Laser System
100 - 1000 mW Output Power
$1,379.00
635 nm

Best Seller!

635 nm Low-Noise Collimated Diode Laser System
5 - 500 mW Output Power
$1,852.00
561 nm
Fanless 561 nm DPSS Laser System
5 - 200 mW Output Power
$2,223.00
635 nm

Best Seller!

635 nm Low-Noise Collimated Diode Laser System with Near-TEM00 Beam
5 - 200 mW Output Power
$2,287.00
405 nm

Best Seller!

405 nm Low-Noise Collimated Diode Laser System
5 - 500 mW Output Power
$2,572.00
635 nm

Best Seller!

635 nm Collimated Diode Laser System
1000 - 1000 mW Output Power
$3,101.00
532 nm

Best Seller!

Fanless Fanless 532 nm DPSS Laser System
500 - 1000 mW Output Power
$3,125.00
532 nm

Best Seller!

532 nm DPSS Laser System
500 - 1000 mW Output Power
$3,125.00
532 nm

Best Seller!

532 nm Low-Noise DPSS Laser System
5 - 200 mW Output Power
$3,296.00
1064 nm

Best Seller!

1064 nm Low-Noise DPSS Laser System
2000 - 2000 mW Output Power
$3,712.00
532 nm

Best Seller!

Fanless 532 nm Single-Longitudinal-Mode DPSS Laser System
5 - 300 mW Output Power
$3,712.00
405 nm

Best Seller!

405 nm Collimated Diode Laser System with TEM00 Beam
5 - 50 mW Output Power
$3,777.00
532 nm

Best Seller!

532 nm DPSS Laser System
2000 mW Output Power
$3,920.00
561 nm
Fanless 561 nm Low-Noise DPSS Laser System
5 - 100 mW Output Power
$3,955.00
405 nm

Best Seller!

405 nm Collimated Diode Laser System
5 - 100 mW Output Power
$4,290.00
532 nm

Best Seller!

Environmentally Sealed 532 nm DPSS Laser System
10 - 300 mW Output Power
$4,446.00
635 nm

Best Seller!

635 nm Collimated Diode Laser System
5 - 30 mW Output Power
$4,806.00
532 nm

Best Seller!

Fanless 532 nm Low-Noise DPSS Laser System
500 - 1000 mW Output Power
$5,118.00
532 nm

Best Seller!

532 nm Low-Noise DPSS Laser System
2000 - 2000 mW Output Power
$6,480.00
1064 nm

Best Seller!

1064 nm Low-Noise DPSS Laser System
4000 - 5000 mW Output Power
$6,927.00
532 nm

Best Seller!

532 nm DPSS Laser System
3000 - 5000 mW Output Power
$7,591.00
635 nm

Best Seller!

635 nm Collimated Diode Laser System
3000 - 6000 mW Output Power
$7,653.00
561 nm
561 nm DPSS Laser System
500 - 1000 mW Output Power
$8,873.00
532 nm

Best Seller!

532 nm DPSS Laser System
- mW Output Power
$9,292.00
1064 nm

Best Seller!

1064 nm DPSS Laser System
10000 - 20000 mW Output Power
$12,151.00
488 nm
488 nm Collimated Diode Laser System with Near-TEM00 Beam
5 - 50 mW Output Power
$12,817.00
488 nm
488 nm Collimated Diode Laser System
5 - 80 mW Output Power
$12,860.00
532 nm

Best Seller!

532 nm DPSS Laser System
8000 - 20000 mW Output Power
$13,204.00
561 nm
561 nm DPSS Laser System
1500 - 2000 mW Output Power
$14,973.00
488 nm
488 nm Low-Noise Collimated Diode Laser System with Near-TEM00 Beam
5 - 50 mW Output Power
$15,801.00
1064 nm

Best Seller!

1064 nm Low-Noise DPSS Laser System
10000 - 20000 mW Output Power
$16,891.00
488 nm
488 nm Collimated Diode Laser System
100 - 150 mW Output Power
$17,740.00
488 nm
488 nm Low-Noise Collimated Diode Laser System
100 - 150 mW Output Power
$21,335.00

Research Papers That Have Used Laserglow Products


Here are a list of research papers that reference Laserglow Technologies products in their Total Internal Reflection Fluorescence Microscopy.

Research Papers

Paper Title Date Published University Product Used In Research
The Yeast INO80 Complex Operates as a Tunable DNA Length-Sensitive Switch to Regulate Nucleosome Sliding Febuary 2018 Department of Biochemistry and Biophysics, University of California, San Francisco 532 nm
3D printable optomechanical cage system with enclosure April 2017 Bethel University, St. Paul, MN, USA 532 nm - 300 mW

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